Users
openSUSE
The openSUSE project uses the MirrorBrain on http://download.opensuse.org/.
The file tree there is about 1.7 Terabyte in size and contains more than 1.200.000 files in ~75.000 directories. The box gets 20.000.000 to 40.000.000 hits a day, i.e. 250-450 per second, and distributes them to more than 150 active mirrors.
Another MirrorBrain instance runs on powerpc.opensuse.org, a separate download server for the distribution of openSUSE builds for the PowerPC architecture. It is a comparably small installation, with only 40.000 files and 5 mirrors.
SourceForge
SourceForge.net doesn't run a complete MirrorBrain setup, but uses parts of the technology to base their mirror selection on Autonomous System Number (ASN) data.
Go-oo
The Go-oo (OpenOffice Development) project uses MirrorBrain since Spring 2008; it can be found at http://go-oo.mirrorbrain.org.
Tree size: 10G, ~3000 files. 13 active mirrors in the database.
openSUSE Education Project
The openSUSE Education project is a community-driven initiative fostering openSUSE in schools. They publish ISO images and package repositories, and use MirrorBrain to deliver them to users. Lars Vogdt, one of the driving forces behind openSUSE Education, is also a committer on the MirrorBrain project.
Linux Administrator Project
The Linux Administrator Project builds and distributes RPM packages through http://download.obs.j0ke.net/.
[planned] OpenOffice.org
OpenOffice.org is evaluating MirrorBrain. Work is underway to deploy it. A preliminary MirrorBrain instance is running at http://openoffice.mirrorbrain.org/ and it is a larger setup, with more than 100 mirrors, and it'll attract heavy traffic. Stay tuned!
[planned] Samba.org
The Samba project is planning to switch to MirrorBrain in the future. A setup is ready on http://samba.mirrorbrain.org/. Stay tuned!